Methods: check ALL you use for FBLD (2/2)
Affinity chromatography, AS-MS, capillary electrophoresis, or ultrafiltration
BLI (biolayer interferometry)
Computational screening
Cryo-EM (cryogenic electron microscopy)
Functional screening (high concentration biochemical, FRET, cell-based, etc.)
ITC (isothermal titration calorimetry)
Literature or known fragments
MS (mass spectrometry, native or covalent)
MST (microscale thermophoresis)
NMR – ligand detected
NMR – protein detected
SPR (surface plasmon resonance)
Thermal shift (or DSF)
X-ray crystallography
Other
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